| 1 | Kinkaid School | Houston, Texas | 16-1-0 | SPC Champions |
| 2 | Sacred Heart Academy | Louisville, Kentucky | 25-3-0 | Region 2 Champions. KHSAA State Champions |
| 3 | Assumption High School | Louisville, Kentucky | 21-6-2 | Region 3 Champions. KHSAA State Runner-Up |
| 4 | St. John's School | Houston, Texas | 14-4-0 | SPC Runner-Up |
| 5 | Villa Duchesne & Oak Hill School | Frontenac, Missouri | 20-2-2 | MidWest Tournament Champions |
| 6 | New Trier High School | Winnetka, Illinois | 22-6-1 | Illinois State Champions |
| 7 | Skyline High School | Ann Arbor, Michigan | 16-2-1 | Michigan Division I State Champions |
| 8 | Ann Arbor Pioneer High School | Pioneer, Michigan | 22-1-0 | Michigan Division I State Runner-Up |
| 9 | Bishop Watterson High School | Columbus, Ohio | 20-0-1 | Ohio State Champions |
| 10 | Scripps Ranch High School | San Diego, California | 19-2-1 | San Diego Section Open Division Champions |
| 11 | Thomas Worthington High School | Worthington, Ohio | 16-3-1 | Ohio State Runner-Up |
| 12 | Torrey Pines High School | San Diego, California | 22-4-0 | San Diego Section Open Division Runner-Up |
| 13 | Christian Academy of Louisville | Louisville, Kentucky | 16-7-0 | Kentucky State Semifinalist |
| 14 | Ballard High School | Louisville, Kentucky | 16-8-0 | Region 1 Champions. KHSAA State Quarterfinalist |
| 15 | Oak Park & River Forest High School | Oak Park, Illinois | 18-6-1 | Illinois State Runner-Up |
| 16 | Lake Forest High School | Lake Forest, Illinois | 20-4-1 | Illinois State Tournament Third Place |
| 17 | Canyon Hills High School | Sain Diego, California | 18-4-0 | San Diego Section Open Division Quarterfinalist |
| 18 | MICDS | Saint Louis, Missouri | 15-5-0 | Midwest Tournament Runner-Up |
| 19 | Los Gatos High School | Los Gatos, California | 16-1-0 | Central Coast Section Champions |
| 20 | New Albany High School | New Albany, Ohio | 12-3-1 | Central Region 2 Runner-Up |
| | | | |
| OC | Canyon Crest Academy | San Diego, California | 19-7-0 | San Diego Section Open Division Semifinalist |
| OC | Columbus Academy | Columbus, Ohio | 14-5-1 | Central 1 Region Runner-Up |
| OC | Glenbrook South High School | Glenview, Illinois | 18-5-0 | Illinois State Tournament Fourth Place |
| OC | Hudson High School | Hudson, Ohio | 15-4-0 | Northeast Region Runner-Up |
| OC | Poway High School | Poway, California | 18-8-1 | San Diego Open Section Semifinalist |
| OC | Saint Francis High School | Mountain View, California | 16-3-1 | Central Coast Section Runner-Up |
| OC | Saline High School | Saline, Michigan | 10-4-1 | Michigan Division I State Semifinalist |
| OC | San Marcos High School | San Marcos, California | 23-5-0 | San Diego Open Section Quarterfinalist |
| OC | Shaker Heights High School | Shaker Heights, Ohio | 15-4-0 | Northeast Region Champions, Ohio State Semifinalist |
2021 WEST/MID-WEST REGION HIGH SCHOOL
