| 1 | Emmaus High School | Emmaus, Pennsylvania | 28-0-0 | District XI AAA Champions, PIAA AAA State Champions |
| 2 | Episcopal Academy | Newtown Square, Pennsylvania | 19-1-0 | Inter-AC Champions, PAISAA Champions |
| 3 | The Hill School | Pottstown, Pennsylvania | 17-2-0 | MAPL Champions, PAISAA Runner-Up |
| 4 | Lower Dauphin High School | Hummelstown, Pennsylvania | 23-1-1 | District III AAA Champions, PIAA AAA State Runner-Up |
| 5 | Wyoming Seminary | Kingston, Pennsylvania | 22-2-0 | District II A Champions, PIAA A State Champions |
| 6 | Twin Valley High School | Elverson, Pennsylvania | 27-2-0 | District III AA Champions, PIAA AA State Champions |
| 7 | Conestoga High School | Berwyn, Pennsylvania | 23-2-0 | District 1 AAA Champions, PIAA AAA State Semifinalist |
| 8 | Gwynedd Mercy Academy High School | Gwynedd Valley, Pennsylvania | 19-3-1 | District 1 AA Champions, PIAA AA State Runner-Up |
| 9 | Southern Lehigh High School | Center Valley, Pennsylvania | 21-1-0 | District XI AA Champions, PIAA AA State Semifinalist |
| 10 | Villa Maria Academy | Malvern, Pennsylvania | 18-4-1 | District 1 AA Runner-Up, PIAA AA State Quarterfinalist |
| 11 | Palmyra Area High School | Palmyra, Pennsylvania | 18-3-1 | District III AA Semifinalist & 3rd Place, PIAA AA State Quarterfinalist |
| 12 | Oley Valley High School | Oley, Pennsylvania | 24-3-0 | District III A Champions, PIAA A State Runner-Up |
| 13 | Mechanicsburg High School | Mechanicsburg, Pennsylvania | 17-7-0 | District III AA Runner-Up, PIAA AA State Semifinalist |
| 14 | Wyoming Area High School | Exeter, Pennsylvania | 19-3-0 | District II A Runner-Up, PIAA A State Semifinalist |
| 15 | Academy of Notre Dame de Namur | Villanova, Pennsylvania | 15-6-0 | PAISAA Semifinalist |
| 16 | Cumberland Valley High School | Mechanicsburg, Pennsylvania | 17-5-0 | District III AAA Runner-Up, PIAA AAA State Quarterfinalist |
| 17 | Crestwood High School | Mountain Top, Pennsylvania | 19-3-0 | District II AA Champions, PIAA AA State First Round |
| 18 | Downingtown West High School | Downingtown, Pennsylvania | 17-6-0 | District 1 AAA Semifinalist & 3rd Place, PIAA AAA State Quarterfinalist |
| 19 | Greenwood High School | Millerstown, Pennsylvania | 19-3-0 | District III A Runner-Up, PIAA A State Quarterfinalist |
| 20 | Merion Mercy Academy | Merion Station, Pennsylvania | 16-6-0 | District 1 AA Semifinalist & 3rd Place, PIAA AA State First Round |
| | | | |
| OC | Central York High School | York, Pennsylvania | 21-6-1 | District III AAA Semifinalist & 3rd Place, PIAA AAA State Semifinalist |
| OC | Garnet Valley High School | Glen Mills, Pennsylvania | 17-5-0 | District 1 AAA Runner-Up, PIAA AAA State First Round |
| OC | Great Valley High School | Malvern, Pennsylvania | 18-5-0 | District 1 AAA Quarterfinalist & 6th Place, PIAA AAA State First Round |
| OC | Methacton High School | Eagleville, Pennsylvania | 18-6-0 | District 1 AAA Semifinalist & 4th Place, PIAA AAA Quarterfinalist |
| OC | Northern York High School | Dillsburg, Pennsylvania | 19-5-0 | District III AA Semifinalist & 4th Place, PIAA AA State First Round |
| OC | Souderton Area High School | Souderton, Pennsylvania | 20-3-0 | District 1 AAA Quarterfinalist & 5th Place, PIAA AAA State First Round |
| OC | Upper Perkiomen High School | Pennsburg, Pennsylvania | 18-4-1 | District 1 AA Semifinalist & 4th Place, PIAA AA First Round |
2021 PENNSYLVANIA REGION HIGH SCHOOL
