| 1 | Delmar | Delmar, DE | 18-0-0 | 1 | Jodi Hollamon | DIAA Division 2 State Champions |
| 2 | Frank W. Cox | Virginia Beach, VA | 20-1-0 | 4 | Taylor Rountree | VHSL Class 6 State Champions, 6A Regional Champions |
| 3 | Trinity Episcopal School | Richmond, VA | 23-0-0 | 3 | Margie Snead | VISAA Division I Champions, LIS Champions |
| 4 | Notre Dame Prep | Towson, MD | 20-1-0 | 5 | Katrina Ross | IAAM A Conference Champions |
| 5 | First Colonial | Virginia Beach, VA | 19-3-0 | 2 | Beanie Schleicher | VHSL Class 6 State Runner-Up (lost to Frank W. Cox 1-0 2OT), 6A Regional Runner-Up |
| 6 | Myers Park | Charlotte, NC | 23-0-0 | 6 | Erica Adcock | NCFHA State Champions |
| 7 | Gloucester | Gloucester Courthouse, VA | 22-0-0 | 7 | Mike Miller | VHSL Class 5 State Champions, 5A Regional Champions |
| 8 | Fairfax | Fairfax, VA | 19-5-0 | 16 | Amber Beaudoin | VHSL Class 6 Semifinalist (lost to Frank W. Cox 0-1 OT SO), 6C Regional Runner-Up (lost to WT Woodson 0-1) |
| 9 | Garrison Forest School | Owings Mills, MD | 16-3-0 | 8 | Traci Davis | IAAM A Conference Runner-Up (lost to NDP 1-3) |
| 10 | Norfolk Academy | Norfolk, VA | 17-6-0 | 11 | Mary Werkheiser | VISAA Division I Semifinalist (lost to Trinity Episcopal 0-2) |
| 11 | South River | Edgewater, MD | 16-3-0 | 10 | Megan Atkinson | MPSSAA 4A State Champions, Anne Arundel County Champions |
| 12 | Cape Henry Collegiate | Virginia Beach, VA | 17-3-0 | 12 | Kate Kinnear | VISAA Division II Champions |
| 13 | Cape Henlopen | Lewes, DE | 17-1-0 | 15 | Kate Austin | DIAA Division I State Champions |
| 14 | Charlotte Latin School | Charlotte, NC | 16-3-0 | 17 | Deb Savino | NCISAA State Champions |
| 15 | Collegiate School | Richmond, VA | 15-7-0 | NR | Karen Doxey | VISAA Division I Runner-Up (lost to Trinity Episcopal 0-3) |
| 16 | C. Milton Wright | Bel Air, MD | 18-1-0 | 20 | Kelly Mattingly | MPSSAA 3A State Champions |
| 17 | St. Catherine's School | Richmond, VA | 11-10-0 | OC | Don Warner | VISAA Division I Semifinalist (lost to Collegiate School 1-2) |
| 18 | James Monroe | Fredericksburg, VA | 18-2-0 | OC | Jamie Tierney | VHSL Class 3 State Champions, 3B Regional Champions |
| 19 | McDonogh School | Owings Mills, MD | 12-3-0 | OC | Denise Wolf | IAAM A Conference Semifinalist (lost to Garrison Forest 1-2 OT) |
| 20 | Archbishop Spalding | Severn, MD | 14-4-0 | 18 | Leslee Brady | IAAM A Conference Semifinalist (lost to NDP 0-1) |
| | | | | | |
| OC | Arundel | Gambrills, MD | 12-5-0 | 19 | Carrie Vosburg | MPSSAA 4A State Quarterfinalist (lost to South River 1-2), Anne Arundel County Runner-Up |
| OC | Broadneck | Annapolis, MD | 10-4-2 | OC | Jen Plack | MPSSAA 4A Regional Semifinalist (lost to South River 2-3) |
| OC | Chantilly | Chantilly, VA | 18-4-0 | NR | Tina Plaugher | VHSL Class 6 State First Round (lost to Fairfax 0-1 OT), 6D Champions |
| OC | Charlotte Country Day | Charlotte, NC | 12-4-2 | OC | Kayleigh Thies | NCISAA State Runner-Up (lost to Charlotte Latin 0-1) |
| OC | Chesapeake | Pasadena, MD | 13-8-0 | OC | Joan Johnson | MPSSAA 3A State Runner-Up (lost to C. Milton Wright 0-3) |
| OC | Deep Run | Glen Allen, VA | 17-3-0 | NR | Rachel Haislip | VHSL Class 5 State Runner-Up (lost to Gloucester 1-2 2OT), 5B Champions |
| OC | Glenelg | Glenelg, MD | 11-2-1 | OC | Nicole Trunzo | MPSSAA 2A State Semifinalist (lost to Liberty 1-2) |
| OC | Langley | McLean, VA | 20-3-0 | 14 | Katie Robinson | VHSL Class 6 Quarterfinalist (lost to First Colonial 1-3), 6D Regional Runner-Up (lost to Chantilly 1-2) |
| OC | Liberty | Eldersburg, MD | 17-2-0 | NR | Brenda Strohmer | MPSSAA 2A State Champions |
| OC | Polytech | Woodside, DE | 13-4-0 | OC | Lynn Richardson | DIAA Division 1 State Semifinalist (lost to Cape Henlopen 0-3) |
| OC | Severna Park | Severna Park, MD | 10-5-1 | OC | Shannon Garden | MPSSAA 4A Regional Semifinalist (lost to Arundel 2-4) |
| OC | W.T. Woodson | Fairfax, VA | 17-2-0 | 9 | Brittany Hopkins | VHSL Class 6 First Round (lost to Langley 1-2), 6C Champions |
