| 1 | Eastern Regional | Voorhees, NJ | Danyle Heilig | 27-0-0 | 1 | Tournament of Champions Winner, NJSIAA Group 4 State Champions, South Group 4 Champions |
| 2 | Hershey | Hershey, PA | Bri Price | 19-5-1 | 15 | PIAA AAA State Champions, District III AAA Semifinalist (lost to Lower Dauphin 0-1), 4th Place through Playback |
| 3 | Donegal | Mount Joy, PA | Jessica Shellenberger | 28-1-0 | 7 | PIAA AA State Champions, District III AA Champions |
| 4 | Oak Knoll School | Summit, NJ | Ali Good | 24-2-0 | 4 | Tournament of Champions Runner-Up (lost to Eastern 1-3), NJSIAA Non-Public State Champions, Non-Public North Champions |
| 5 | Delmar | Delmar, DE | Jodi Hollamon | 18-0-0 | 5 | DIAA Division 2 State Champions |
| 6 | Lower Dauphin | Hummelstown, PA | Linda Kreiser | 22-2-1 | 11 | PIAA AAA State Runner-Up (lost to Hershey 1-2 OT), District III AAA Champions |
| 7 | Frank W. Cox | Virginia Beach, VA | Taylor Rountree | 20-1-0 | 14 | VHSL Class 6 State Champions, 6A Regional Champions |
| 8 | Sacred Heart Academy | Louisville, KY | Eleanor Cowley Mitchell | 25-1-0 | 10 | KHSAA State Champions |
| 9 | Trinity Episcopal School | Richmond, VA | Margie Snead | 23-0-0 | 9 | VISAA Division I Champions, LIS Champions |
| 10 | Wyoming Seminary | Kingston, PA | Karen Klassner | 21-2-1 | 16 | PIAA A State Champions, District II A Champions |
| 11 | Garden City | Garden City, NY | Diane Chapman | 19-0-0 | NR | NYS Class B State Champions, Nassau County Class B Champions |
| 12 | Episcopal Academy | Newtown Square, PA | Gina Buggy | 15-2-2 | 21 | PAISAA Champions |
| 13 | Lakeland | Shrub Oak, NY | Sharon Sarsen | 22-1-0 | 3 | NYS Class B State Runner-Up (lost to Garden City 0-1), NYS Section I B Champions |
| 14 | Emmaus | Emmaus, PA | Sue Butz-Stavin | 26-1-0 | 2 | PIAA AAA State Semifinalist (lost to Hershey 3-5), EPC Champions, District XI AAA Champions |
| 15 | Moorestown | Moorestown, NJ | Ali Collins | 22-2-1 | 20 | Tournament of Champions Semifinalist (lost to Oak Knoll 0-1), NJSIAA Group 3 State Champions, Central Group 3 Champions |
| 16 | Palmyra Area | Palmyra, PA | Kent Harshman | 19-6-1 | NR | PIAA AA State Runner-Up (lost to Donegal 0-1 OT), District III AA Runner-Up (lost to Donegal 0-3) |
| 17 | New Trier | Winnetka, IL | Stephanie Nykaza | 22-2-0 | 17 | Illinois State Champions |
| 18 | Penn Manor | Millersville, PA | Matt Soto | 22-3-1 | 6 | PIAA AAA State Quarterfinalist (lost to Hershey 0-1), District III AAA Runner-Up (lost to Lower Dauphin 0-2) |
| 19 | Staples | Westport, CT | Ian Tapsall | 22-1-0 | 22 | CIAC Class L State Champions, FCIAC Runner-Up (lost to Darien 1-2) |
| 20 | Columbus Academy | Columbus, OH | Anne Horton | 21-0-0 | 18 | Ohio State Champions |
| 21 | Phillips Andover Academy | Andover, MA | Kate Dolan | 20-0-0 | 19 | NEPSAC A Champions |
| 22 | Notre Dame Prep | Towson, MD | Katrina Ross | 20-1-0 | NR | IAAM A Conference Champions |
| 23 | Somerset Berkley Regional | Somerset, MA | Jen Crook | 23-1-0 | NR | MIAA Division I State Champions, MIAA South Division I Champions |
| 24 | First Colonial | Virginia Beach, VA | Beanie Schleicher | 19-3-0 | 8 | VHSL Class 6 State Runner-Up (lost to Frank W. Cox 1-0 2OT), 6A Regional Runner-Up |
| 25 | Los Gatos | Los Gatos, CA | Henry Reyes | 22-0-0 | 24 | Central Coast Section Champions |
2018 HIGH SCHOOL FINAL
